I got the same message as well, but I have never set a region for either of my drives.
Both were installed after AnyDVD was installed on the computer, and it was enabled at first boot up with the new drives. Both have always played anything thrown at them, as long as AnyDVD was running, so I never felt a need to set the region. (keeping all remaining changes, as if the drive were new)
However, this is the first time this message has ever emerged, after countless backups.
My disk backed up as well, with 22.214.171.124, without setting the region, and backup plays fine.
I enabled CSS archive, and after the initial backup, trying again results in the message only appearing once, when the disk is inserted, where previously, it appeared a second time when choosing the source path for CloneDVD2.