For Error 25001 try this,
Browse to HKEY_LOCAL_MACHINE\Software\Microsoft. Right-click on Driver Signing and click permissions. Then click add and type Administrators then click ok. Make sure Administrators have full control checked.
Even though under your permissions tab under driver signing you set full control and read and have them checked look under the advanced tab for special permissions and give administrator full control.
You should make sure you are running as an administrator when installing alcohol.
Make sure you reboot.
Also next time you install Alcohol do it in expert mode and change the names for Busport and Miniport to something different. This will make sure that your old installation dosn't interfere with this one.